{"id":3138,"date":"2021-10-28T14:19:59","date_gmt":"2021-10-28T12:19:59","guid":{"rendered":"https:\/\/binwit.pl\/en\/?page_id=3138"},"modified":"2021-10-28T20:41:29","modified_gmt":"2021-10-28T18:41:29","slug":"transmission-electron-microscopy-at-hirszfeld-institute","status":"publish","type":"page","link":"https:\/\/binwit.pl\/en\/transmission-electron-microscopy\/transmission-electron-microscopy-at-hirszfeld-institute\/","title":{"rendered":"Transmission Electron Microscopy at Hirszfeld Institute"},"content":{"rendered":"\n<div class=\"wp-block-qubely-row qubely-section qubely-block-6c8d0a\"><div class=\"qubely-row-overlay\"><\/div><div class=\"qubely-container\"><div class=\"qubely-row \">\n<div class=\"wp-block-qubely-column qubely-column qubely-column-front qubely-block-036e85\"><div class=\"qubely-column-inner\">\n<div class=\"wp-block-qubely-heading qubely-block-ab16a5\" id=\"1-stosowane-techniki-\"><div class=\"qubely-block-heading  \"><div class=\"qubely-heading-container\"><h2 class=\"qubely-heading-selector\">Techniques <\/h2><\/div><\/div><\/div>\n\n\n\n<ol class=\"wp-block-list\" start=\"1\"><li>Biological samples imaging using transmissive electron microscopy (<strong>TEM<\/strong>).<\/li><li><strong>Cryo EM<\/strong> cryogenic imaging using transmissive electron microscopy.<\/li><li>Energy dispersive X-ray spectroscopy \u2013 element sample analysis.<\/li><li>Ultrastructure samples creation in both room temperature and cryogenic conditions.<\/li><li><strong>Immunogold<\/strong> labeling.<\/li><\/ol>\n\n\n\n<div class=\"wp-block-qubely-heading qubely-block-d5a239\" id=\"2-aparatura-\"><div class=\"qubely-block-heading  \"><div class=\"qubely-heading-container\"><h2 class=\"qubely-heading-selector\">Laboratory equipment <\/h2><\/div><\/div><\/div>\n\n\n\n<ol class=\"wp-block-list\" start=\"1\"><li><span style=\"color: #012f53;\" data=\"#012f53\" class=\"qubely-text-has-color\"><strong>JEOL 1200 EX<\/strong><\/span> microscope,<\/li><li><strong><span style=\"color: #012f53;\" data=\"#012f53\" class=\"qubely-text-has-color\">JEOL JEM F-200<\/span><\/strong> microscope,<ul><li>Cold field emission with maximum accelerating voltage of <strong>200 kV<\/strong>,<\/li><li>CR pole piece, with maximum available contrast and low contamination parameter, dedicated for low temperature and general biological imaging,<\/li><li><strong><span style=\"color: #012f53;\" data=\"#012f53\" class=\"qubely-text-has-color\">EDS JEOL CENTURIO DRY SD30GV<\/span><\/strong> detector<\/li><li><strong>STEM<\/strong> microscope mode with Bright Field (<strong>BF<\/strong>) detectors and High-Angle Annular Dark-Field (<strong>HAADF<\/strong>),<\/li><li><strong><span style=\"color: #012f53;\" data=\"#012f53\" class=\"qubely-text-has-color\">CMOS TVIPS TemCam-XF416<\/span><\/strong> camera with 16 MPix resolution and 5 x 63.5 mm<sup>2<\/sup> area,<\/li><li>High tilt handle for room temperature tomography,<\/li><li><strong><span style=\"color: #012f53;\" data=\"#012f53\" class=\"qubely-text-has-color\">FISCHIONE 2550 Cryo-Transfer<\/span><\/strong> low temperature handle with tomography capabilities.<\/li><\/ul><\/li><li><strong><span style=\"color: #012f53;\" data=\"#012f53\" class=\"qubely-text-has-color\">Leica EM UC7<\/span><\/strong> microtome with <strong><strong><span style=\"color: #012f53;\" data=\"#012f53\" class=\"qubely-text-has-color\">FC7<\/span><\/strong><\/strong> low temperature sectioning syste,<\/li><li>Leica Automatic Plunge Freezer<strong><span style=\"color: #012f53;\" data=\"#012f53\" class=\"qubely-text-has-color\"> EM GP2<\/span><\/strong>,<\/li><li><strong><span style=\"color: #012f53;\" data=\"#012f53\" class=\"qubely-text-has-color\">Quorum Technologies Turbo-Pumped Thermal Evaporator<\/span><\/strong> <strong><span style=\"color: #012f53;\" data=\"#012f53\" class=\"qubely-text-has-color\">K975X<\/span><\/strong>,<\/li><li>Plazma cleaner <strong><span style=\"color: #012f53;\" data=\"#012f53\" class=\"qubely-text-has-color\">Targeo-EM<\/span><\/strong>,<\/li><li><strong><span style=\"color: #012f53;\" data=\"#012f53\" class=\"qubely-text-has-color\">EMP 5160<\/span><\/strong> Tissue Processor.<\/li><\/ol>\n\n\n\n<div class=\"wp-block-qubely-heading qubely-block-b1471e\" id=\"3-publikacje-\"><div class=\"qubely-block-heading  \"><div class=\"qubely-heading-container\"><h2 class=\"qubely-heading-selector\">Publications<\/h2><\/div><\/div><\/div>\n\n\n\n<ol class=\"wp-block-list\" start=\"1\"><li>Markowski A, Migda\u0142 P, Zygmunt A, Zaremba-Czogalla M, Gubernator J Evaluation of the in vitro cytotoxic activity of Ursolic Acid PLGA Nanoparticles against Pancreatic Ductal Adenocarcinoma Cell Lines Materials 14 (71), 4917 (2021) <a href=\"https:\/\/doi.org\/10.3390\/ma14174917\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/doi.org\/10.3390\/ma14174917<\/a><\/li><\/ol>\n\n\n\n<div class=\"wp-block-qubely-heading qubely-block-5d506a\" id=\"4-kontakt-\"><div class=\"qubely-block-heading  \"><div class=\"qubely-heading-container\"><h2 class=\"qubely-heading-selector\">Contact <\/h2><\/div><\/div><\/div>\n\n\n\n<p>Pawe\u0142 Migda\u0142 Ph. D. <a href=\"mailto:pawel.migdal@hirszfeld.pl\">pawel.migdal@hirszfeld.pl<\/a><\/p>\n\n\n\n<p>Krzysztof Pawlik Ph. D. <a href=\"mailto:krzysztof.pawlik@hirszfeld.pl\">krzysztof.pawlik@hirszfeld.pl<\/a><\/p>\n\n\n\n<div class=\"wp-block-qubely-heading qubely-block-9f5f6a\" id=\"5-galeria\"><div class=\"qubely-block-heading  \"><div class=\"qubely-heading-container\"><h2 class=\"qubely-heading-selector\">Gallery<\/h2><\/div><\/div><\/div>\n\n\n<style type=\"text\/css\">\n#foogallery-gallery-3141.fg-masonry { --fg-gutter: 10px; }<\/style>\n\t\t\t<div class=\"foogallery foogallery-container foogallery-masonry foogallery-lightbox-foobox fg-center fg-masonry fg-ready fg-dark fg-shadow-small fg-loading-default fg-loaded-fade-in fg-hover-grayscale fg-hover-fade fg-hover-eye fg-col3\" id=\"foogallery-gallery-3141\" data-foogallery=\"{&quot;item&quot;:{&quot;showCaptionTitle&quot;:false,&quot;showCaptionDescription&quot;:false},&quot;lazy&quot;:true}\" style=\"--fg-title-line-clamp: 0; --fg-description-line-clamp: 0;\" >\n\t\t<div class=\"fg-column-width\"><\/div>\n\t<div class=\"fg-gutter-width\"><\/div>\n\t\t<div class=\"fg-item fg-type-image fg-idle\"><figure class=\"fg-item-inner\"><a href=\"https:\/\/binwit.pl\/en\/wp-content\/uploads\/sites\/2\/2021\/10\/IMG_7610-900px.jpg\" data-attachment-id=\"3149\" data-type=\"image\" class=\"fg-thumb\"><span class=\"fg-image-wrap\"><img fetchpriority=\"high\" decoding=\"async\" width=\"250\" height=\"375\" class=\"skip-lazy fg-image\" data-src-fg=\"https:\/\/binwit.pl\/en\/wp-content\/uploads\/sites\/2\/cache\/2021\/10\/IMG_7610-900px\/3157822310.jpg\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%22http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%22%20width%3D%22250%22%20height%3D%22375%22%20viewBox%3D%220%200%20250%20375%22%3E%3C%2Fsvg%3E\" loading=\"eager\"><\/span><span class=\"fg-image-overlay\"><\/span><\/a><\/figure><div class=\"fg-loader\"><\/div><\/div><div class=\"fg-item fg-type-image fg-idle\"><figure class=\"fg-item-inner\"><a href=\"https:\/\/binwit.pl\/en\/wp-content\/uploads\/sites\/2\/2021\/10\/Image-2021-10-19-at-12.29.50.jpeg\" data-attachment-id=\"3148\" data-type=\"image\" class=\"fg-thumb\"><span class=\"fg-image-wrap\"><img decoding=\"async\" width=\"250\" height=\"445\" class=\"skip-lazy fg-image\" data-src-fg=\"https:\/\/binwit.pl\/en\/wp-content\/uploads\/sites\/2\/cache\/2021\/10\/Image-2021-10-19-at-12_29_50\/1125057951.jpeg\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%22http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%22%20width%3D%22250%22%20height%3D%22445%22%20viewBox%3D%220%200%20250%20445%22%3E%3C%2Fsvg%3E\" loading=\"eager\"><\/span><span class=\"fg-image-overlay\"><\/span><\/a><\/figure><div class=\"fg-loader\"><\/div><\/div><div class=\"fg-item fg-type-image fg-idle\"><figure class=\"fg-item-inner\"><a href=\"https:\/\/binwit.pl\/en\/wp-content\/uploads\/sites\/2\/2021\/10\/Image-2021-10-19-at-12.26.01.jpeg\" data-attachment-id=\"3145\" data-type=\"image\" class=\"fg-thumb\"><span class=\"fg-image-wrap\"><img decoding=\"async\" width=\"250\" height=\"445\" class=\"skip-lazy fg-image\" data-src-fg=\"https:\/\/binwit.pl\/en\/wp-content\/uploads\/sites\/2\/cache\/2021\/10\/Image-2021-10-19-at-12_26_01\/1922018992.jpeg\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%22http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%22%20width%3D%22250%22%20height%3D%22445%22%20viewBox%3D%220%200%20250%20445%22%3E%3C%2Fsvg%3E\" loading=\"eager\"><\/span><span class=\"fg-image-overlay\"><\/span><\/a><\/figure><div class=\"fg-loader\"><\/div><\/div><div class=\"fg-item fg-type-image fg-idle\"><figure class=\"fg-item-inner\"><a href=\"https:\/\/binwit.pl\/en\/wp-content\/uploads\/sites\/2\/2021\/10\/Image-2021-10-19-at-12.23.51.jpeg\" data-attachment-id=\"3142\" data-type=\"image\" class=\"fg-thumb\"><span class=\"fg-image-wrap\"><img decoding=\"async\" width=\"250\" height=\"445\" class=\"skip-lazy fg-image\" data-src-fg=\"https:\/\/binwit.pl\/en\/wp-content\/uploads\/sites\/2\/cache\/2021\/10\/Image-2021-10-19-at-12_23_51\/1079742815.jpeg\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%22http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%22%20width%3D%22250%22%20height%3D%22445%22%20viewBox%3D%220%200%20250%20445%22%3E%3C%2Fsvg%3E\" loading=\"eager\"><\/span><span class=\"fg-image-overlay\"><\/span><\/a><\/figure><div class=\"fg-loader\"><\/div><\/div><div class=\"fg-item fg-type-image fg-idle\"><figure class=\"fg-item-inner\"><a href=\"https:\/\/binwit.pl\/en\/wp-content\/uploads\/sites\/2\/2021\/10\/Image-2021-10-19-at-12.25.26.jpeg\" data-attachment-id=\"3144\" data-type=\"image\" class=\"fg-thumb\"><span class=\"fg-image-wrap\"><img decoding=\"async\" width=\"250\" height=\"141\" class=\"skip-lazy fg-image\" data-src-fg=\"https:\/\/binwit.pl\/en\/wp-content\/uploads\/sites\/2\/cache\/2021\/10\/Image-2021-10-19-at-12_25_26\/2083297873.jpeg\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%22http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%22%20width%3D%22250%22%20height%3D%22141%22%20viewBox%3D%220%200%20250%20141%22%3E%3C%2Fsvg%3E\" loading=\"eager\"><\/span><span class=\"fg-image-overlay\"><\/span><\/a><\/figure><div class=\"fg-loader\"><\/div><\/div><div class=\"fg-item fg-type-image fg-idle\"><figure class=\"fg-item-inner\"><a href=\"https:\/\/binwit.pl\/en\/wp-content\/uploads\/sites\/2\/2021\/10\/Image-2021-10-19-at-12.25.00.jpeg\" data-attachment-id=\"3143\" data-type=\"image\" class=\"fg-thumb\"><span class=\"fg-image-wrap\"><img decoding=\"async\" width=\"250\" height=\"445\" class=\"skip-lazy fg-image\" data-src-fg=\"https:\/\/binwit.pl\/en\/wp-content\/uploads\/sites\/2\/cache\/2021\/10\/Image-2021-10-19-at-12_25_00\/1201516637.jpeg\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%22http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%22%20width%3D%22250%22%20height%3D%22445%22%20viewBox%3D%220%200%20250%20445%22%3E%3C%2Fsvg%3E\" loading=\"eager\"><\/span><span class=\"fg-image-overlay\"><\/span><\/a><\/figure><div class=\"fg-loader\"><\/div><\/div><div class=\"fg-item fg-type-image fg-idle\"><figure class=\"fg-item-inner\"><a href=\"https:\/\/binwit.pl\/en\/wp-content\/uploads\/sites\/2\/2021\/10\/Image-2021-10-19-at-12.27.01.jpeg\" data-attachment-id=\"3146\" data-type=\"image\" class=\"fg-thumb\"><span class=\"fg-image-wrap\"><img decoding=\"async\" width=\"250\" height=\"141\" class=\"skip-lazy fg-image\" data-src-fg=\"https:\/\/binwit.pl\/en\/wp-content\/uploads\/sites\/2\/cache\/2021\/10\/Image-2021-10-19-at-12_27_01\/3285257968.jpeg\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%22http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%22%20width%3D%22250%22%20height%3D%22141%22%20viewBox%3D%220%200%20250%20141%22%3E%3C%2Fsvg%3E\" loading=\"eager\"><\/span><span class=\"fg-image-overlay\"><\/span><\/a><\/figure><div class=\"fg-loader\"><\/div><\/div><div class=\"fg-item fg-type-image fg-idle\"><figure class=\"fg-item-inner\"><a href=\"https:\/\/binwit.pl\/en\/wp-content\/uploads\/sites\/2\/2021\/10\/Image-2021-10-19-at-12.29.22.jpeg\" data-attachment-id=\"3147\" data-type=\"image\" class=\"fg-thumb\"><span class=\"fg-image-wrap\"><img decoding=\"async\" width=\"250\" height=\"141\" class=\"skip-lazy fg-image\" data-src-fg=\"https:\/\/binwit.pl\/en\/wp-content\/uploads\/sites\/2\/cache\/2021\/10\/Image-2021-10-19-at-12_29_22\/238119667.jpeg\" src=\"data:image\/svg+xml,%3Csvg%20xmlns%3D%22http%3A%2F%2Fwww.w3.org%2F2000%2Fsvg%22%20width%3D%22250%22%20height%3D%22141%22%20viewBox%3D%220%200%20250%20141%22%3E%3C%2Fsvg%3E\" loading=\"eager\"><\/span><span class=\"fg-image-overlay\"><\/span><\/a><\/figure><div class=\"fg-loader\"><\/div><\/div><\/div><\/div><\/div>\n\n\n\n<div class=\"wp-block-qubely-column qubely-column qubely-column-front qubely-block-ea73d3\"><div class=\"qubely-column-inner\">\n<div class=\"wp-block-qubely-table-of-contents qubely-block-83db3b qubely-block-table-of-contents qubely-align-left\" data-qubelyanimation=\"{&quot;animation&quot;:&quot;fade&quot;,&quot;name&quot;:&quot;fadeCenter&quot;,&quot;repeat&quot;:&quot;once&quot;,&quot;direction&quot;:&quot;Center&quot;,&quot;duration&quot;:1000,&quot;delay&quot;:0,&quot;curve&quot;:&quot;ease-in-out&quot;,&quot;openAnimation&quot;:1}\"><div class=\"qubely-table-of-contents\" data-scroll-offset=\"20\"><div class=\"qubely-table-of-contents-header qubely-toc-header-frontend qubely-justify-between\"><div class=\"qubely-table-of-contents-heading\"><div class=\"title\"><strong>Contents:<\/strong><\/div><\/div><\/div><div class=\"qubely-table-of-contents-body\"><ol class=\"ordered-list\"><li><a href=\"#1-stosowane-techniki-\">Techniques <\/a><\/li><li><a href=\"#2-aparatura-\">Laboratory equipment <\/a><\/li><li><a href=\"#3-publikacje-\">Publications<\/a><\/li><li><a href=\"#4-kontakt-\">Contact <\/a><\/li><li><a href=\"#5-galeria\">Gallery<\/a><\/li><\/ol><\/div><a href=\"#\" class=\"qubely-back-to-top-button fas fa-angle-up\"><\/a><\/div><\/div>\n<\/div><\/div>\n<\/div><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":7,"featured_media":0,"parent":2736,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"qubely_global_settings":"","qubely_interactions":"","_uag_custom_page_level_css":"","footnotes":""},"class_list":["post-3138","page","type-page","status-publish","hentry","clearfix"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"qubely_landscape":false,"qubely_portrait":false,"qubely_thumbnail":false,"ultp_layout_landscape_large":false,"ultp_layout_landscape":false,"ultp_layout_portrait":false,"ultp_layout_square":false,"post-thumbnail":false},"uagb_author_info":{"display_name":"Redaktor","author_link":"https:\/\/binwit.pl\/en\/author\/tarraya\/"},"uagb_comment_info":0,"uagb_excerpt":null,"_links":{"self":[{"href":"https:\/\/binwit.pl\/en\/wp-json\/wp\/v2\/pages\/3138","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/binwit.pl\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/binwit.pl\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/binwit.pl\/en\/wp-json\/wp\/v2\/users\/7"}],"replies":[{"embeddable":true,"href":"https:\/\/binwit.pl\/en\/wp-json\/wp\/v2\/comments?post=3138"}],"version-history":[{"count":3,"href":"https:\/\/binwit.pl\/en\/wp-json\/wp\/v2\/pages\/3138\/revisions"}],"predecessor-version":[{"id":3154,"href":"https:\/\/binwit.pl\/en\/wp-json\/wp\/v2\/pages\/3138\/revisions\/3154"}],"up":[{"embeddable":true,"href":"https:\/\/binwit.pl\/en\/wp-json\/wp\/v2\/pages\/2736"}],"wp:attachment":[{"href":"https:\/\/binwit.pl\/en\/wp-json\/wp\/v2\/media?parent=3138"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}