Transmission Electron Microscopy at Hirszfeld Institute

1. Biological samples imaging using transmissive electron microscopy (TEM).
2. Cryo EM cryogenic imaging using transmissive electron microscopy.
3. Energy dispersive X-ray spectroscopy – element sample analysis.
4. Ultrastructure samples creation in both room temperature and cryogenic conditions.
5. Immunogold labeling.

1. JEOL 1200 EX microscope,
2. JEOL JEM F-200 microscope,
   • Cold field emission with maximum accelerating voltage of 200 kV,
   • CR pole piece, with maximum available contrast and low contamination parameter, dedicated for low temperature and general biological imaging,
   • EDS JEOL CENTURIO DRY SD30GV detector
   • STEM microscope mode with Bright Field (BF) detectors and High-Angle Annular Dark-Field (HAADF),
   • CMOS TVIPS TemCam-XF416 camera with 16 MPix resolution and 5 x 63.5 mm² area,
   • High tilt handle for room temperature tomography,
   • FISCHIONE 2550 Cryo-Transfer low temperature handle with tomography capabilities.
3. Leica EM UC7 microtome with FC7 low temperature sectioning syste,
4. Leica Automatic Plunge Freezer EM GP2,
5. Quorum Technologies Turbo-Pumped Thermal Evaporator K975X,
6. Plazma cleaner Targeo-EM,
7. EMP 5160 Tissue Processor.

1. Markowski A, Migdał P, Zygmunt A, Zaremba-Czogalla M, Gubernator J Evaluation of the in vitro cytotoxic activity of Ursolic Acid PLGA Nanoparticles against Pancreatic Ductal Adenocarcinoma Cell Lines Materials 14 (71), 4917 (2021) https://doi.org/10.3390/ma14174917

Paweł Migdał Ph. D. pawel.migdal@hirszfeld.pl

Krzysztof Pawlik Ph. D. krzysztof.pawlik@hirszfeld.pl